CD5 anticorps (Biotin)

N° du produit ABIN6184967

Cet anticorps anti-CD5 est un anticorps Souris Monoclonal détectant CD5 dans FACS, IF et IHC (f). Adapté pour Humain, Rat, Boeuf (Vache) et Lapin.

Aperçu rapide pour CD5 anticorps (Biotin) (ABIN6184967)

Antigène: CD5

Reactivité: Humain, Rat, Boeuf (Vache), Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD5 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C5-473 CD5-54-F6

Détail du produit anti-CD5 anticorps (Biotin)

Fonction: Mouse Monoclonal anti-CD5 (C5/473 + CD5/54/F6), Biotin Conjugate

Attributs du produit: This antibody recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma ( ) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human CD5 recombinant protein (C5/473), A synthetic peptide from the intracellular region of human CD5 (CD5/54/F6)

Isotype: IgG

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

293T, Ramos, or MOLT-4 Cells. Tonsil.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur CD5

Antigène: CD5

Autre désignation: CD5

Sujet: CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5

Poids moléculaire: 67 kDa

ID gène: 921, 58685

UniProt: P06127